The IS0 12099:2017 standard is a guideline for applying NIR spectroscopy in the agricultural industries for analysis of grain, feed ingredients, finished feed and petfood products. NIR instruments are widely used in these industries for determination of constituents such as moisture, fat, protein, starch, fibers and more for quality and process control purposes.
The standard includes technical requirements for an NIR instrument:
- Using the Near Infrared wavelength region
- Including diagnostic test systems for testing photometric noise and reproducibility, as well as wavelength accuracy and precision.
- Measurement of a large sample surface to eliminate sample inhomogeneity influences.
The standard is focused on guidelines for the procedures and calculations required for validation of NIR calibrations. It includes recommendations on the number of validation samples to use as well as other considerations to take into account when selecting representative validation samples.
Perten’s DA 7250 NIR Analyzer is an example of an instrument which fully complies with the standard. The instrument uses internal automatic white referencing, an internal Xenon lamp for wavelength verifications, and can be used with external check cells. Measurements are done in the Near Infrared region 950 – 1650 nm measuring the whole surface of a large rotating open sample dish.
Perten Instrument’s web based NetPlus software includes validation modules which makes it very easy to perform the validation calculations needed for compliance with the standard, such as calculations of bias, slope and standard errors of prediction against the reference methods.
A comprehensive glossary with explanations of various definitions in NIR spectroscopy is included in the standard. This is very useful in making sure terms are used and understood in the same ways.
The ISO 12099 is a valuable tool both for users of individual NIR instruments as well as maintenance of NIR instruments in networks where control samples are used for instrument alignments and performance checks.